A paper-based sensor to detect antimicrobial resistance triggering pollutants

New Delhi: Indian Institute of Technology Madras and United Kingdom researchers have developed a paper-based sensor that can detect antimicrobial pollutants, which induce antimicrobial resistance in water bodies. This sensor works on a ‘see and tell’ mechanism that makes it logistically effective for wide implementation.

Scientific communities across the world are focused on Antimicrobial Resistance (AMR), which could possibly become a worldwide health crisis involving deadly pathogens. Water bodies are the major source for the dissemination and transfer of AMR. Periodic monitoring of antimicrobial pollutants and antibiotic-resistant genes is the key to assess the current situation of AMR in India.

In these conditions, low-cost and field-deployable sensors to detect pollutants in water bodies could be a viable tool for environmental surveillance.

A video explaining this research can be downloaded from https://fromsmash.com/IIT-Madras-Video (Valid till 11th May 2021). This research was first reported through a journal publication in Nature Scientific Reports and was acclaimed as one of the top 100 in chemistry.

This research was funded by the Department of Science and Technology (DST), Government of India in bilateral collaboration with the UK’s Natural Environment Research Council and Engineering and Physical Sciences Research Council (EPSRC) under the ‘Indo UK Water Quality Research Programme.’

In IIT Madras, this research was led by Prof. S. Pushpavanam, Institute Chair Professor, Department of Chemical Engineering, IIT Madras and Dr. T. Renganathan, Associate Professor, Department of Chemical Engineering, IIT Madras.

Elaborating on the unique aspects of this research, Prof. S. Pushpavanam, Institute Chair Professor, Department of Chemical Engineering, IIT Madras, said, “Paper-based sensor offers an affordable platform for various point-of-care applications as they support fluid flow based on a wicking action and governed by capillary forces. This eliminates the requirement of a pump-to-flow liquids. We have come up with a novel method for the fabrication of paper- based devices using a commercial laser printer.”

Further, speaking about the technical aspects of this sensor, Prof. Pushpavanam, said, “The way it works is that we use a porous substrate such as paper, which enables us to use standard software to print required designs on it. Once printed, the printer ink is deposited on the surface of the paper. When heated this penetrates the thickness of the paper and forms a hydrophobic barrier through which liquid cannot pass. This allows us to direct the flow of liquid in preferential directions through the areas which are not printed and are hydrophilic.”

The practical applications of these sensors include environmental monitoring, food safety analysis, and healthcare monitoring.

Speaking about the current Indo-UK project, Dr. T. Renganathan, Associate Professor, Department of Chemical Engineering, IIT Madras, said, “We have used these fabricated devices for the detection of antibiotics such as ciprofloxacin, biocides such as triclosan and heavy metals such as chromium, copper and lead. These devices can be used for antimicrobial resistance surveillance in water bodies.”

Highlighting how this sensor is unique compared to existing technology, Dr. T. Renganathan added, “We use a normal laser printer without any modification and it offers high resolution and accuracy. The hydrophobic barriers are compatible against organic solvents and high temperature. The developed laser printed paper-based microfluidic sensor is a viable option for large scale manufacturing and enables routine monitoring of pollutants in both developed and resource constrained regions.”

The novel strategy for low-cost fabrication of the robust Laser Printed-Microfluidic Paper-Based Analytical Sensors developed by IIT Madras will help to detect antimicrobials easily in the parts per million range. It will also help understand the relationship between AMR and AMR-triggering pollutants and assist policymakers in framing solutions to tackle grand societal AMR challenge.

The novel strategy of combining adsorption based pre-concentration using reagents that undergo a measurable colour change enabled parts per billion level detection of pollutants. The process utilizes the easily available laser printer and hence offers tremendous potential for large scale sensor fabrication. It could enable community-driven microfluidics and facilitate mass surveillance.